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Objective:To investigate the relationship of mitochondrial DNA (mtDNA) copy number with clinicopathologic characteristics and its influence on the prognosis of hepatocellular carcinoma (HCC) patients.Methods:The retrospective case-control study was conducted. The clinicopathological data of 71 HCC patients undergoing surgical treatment in the Eastern Hepatobiliary Surgery Hospital of Naval Medical University from March to June 2011 were collected. There were 61 males and 10 females, aged from 26 to 80 years, with a median age of 55 years. The mtDNA copy number of tumor tissues and adjacent normal tissues were measured for all patients. Observation indicators: (1) the mtDNA copy number of tumor tissues and adjacent normal tissues and relationship between the mtDNA copy number and clinicopathological characteristics of HCC patients; (2) follow-up; (3) related factors for the prognosis of HCC patients. Follow-up using outpatient examination or telephone interview was conducted to detect postoperative survival of patients up to September 2019. Measurement data with normal distribution were described as Mean± SD, and comparison between groups was analyzed using independent samples t test or the matched samples t test. Measurement data with skewed distribution were described as M(range). Count data were represented as absolute numbers, and comparison between groups was analyzed using the chi-square test or Fisher exact probability. Univariate and multivariate analyses were conducted using the COX regressional model. Variables with P<0.10 in the univariate analysis were included for the multivariate analysis. Survival rates were calculated using the Kaplan-Meier method, and Log-rank test was used for survival analysis. Results:(1) The mtDNA copy number of tumor tissues and adjacent normal tissues and relationship between the mtDNA copy number and clinicopathological characteristics of HCC patients: of 71 HCC patients, the mtDNA copy number was 0.85±0.08 in tumor tissues, versus 1.16±0.08 in adjacent normal tissues, showing a significant difference between them ( t=2.96, P<0.05). Of 71 HCC patients, 48 cases were mtDNA-low and 23 cases were mtDNA-high. Cases with tumor capsule as integrity or not-integrity, cases with or without microvascular (MVI) in mtDNA-low and mtDNA-high patients were 20, 28, 21, 27 and 16, 7, 4, 19, respectively, showing significant differences ( χ2=4.84, 4.74, P<0.05). (2) Follow-up: 71 patients were followed up for 2.1 to 85.3 months, with a median follow-up time of 47.8 months. The 1-, 3-, 5-year overall survival rates of 71 HCC patients were 87.3%, 64.7, 37.4%, respectively. Moreover, the 1-, 3-, 5-year overall survival rates were 81.2%, 50.0%, 29.2% of the mtDNA-low patients, versus 95.7%, 86.5%, 54.7% of the mtDNA-high patients, showing a significant difference between the two groups ( χ2=5.86, P<0.05). (3) Related factors for the prognosis of HCC patients. Results of univariate analysis showed that the number of tumor, portal vein tumor thrombus, MVI, Barcelona Clinic Liver Cancer stage, mtDNA copy number were related factors for the prognosis of HCC patients ( hazard ratios=2.211, 2.911, 3.899, 3.587, 0.440, 95% confidence intervals as 1.024?4.777, 1.485?5.704, 2.115?7.186, 1.615?7.966, 0.223?0.871, P<0.05). Results of multivariate analysis showed that MVI and mtDNA copy number were independent influencing factors for the prognosis of HCC patients ( hazard ratios=2.754, 0.437, 95% confidence intervals as 1.374?5.521, 0.205?0.932, P<0.05). Conclusions:The mtDNA copy number of HCC patients is related with tumor capsule and MVI. The mtDNA copy number and MVI are independent influencing factors for the prognosis of HCC patients.
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Objective To observe the clinical safety and prognosis effect of laparoscopic cholecystectomy in the treatment of acute cholecystitis complicating gallstones.Methods Seventy cases of acute cholecystitis complicating gallstones treated by laparoscopic cholecystectomy in the hospital from December 2013 to October 2016 were retrospectively analyzed,and randomly divided into experimental group and control group.The patients in the control group received clinical open surgery,while the experimental group received laparoscopic surgical treatment.The operative effect,postoperative recovery and complication occurrence rate were observed in the two groups.Results The operation time,intraoperative bleeding volume and hospitalization time in the experimental group were significantly better than those in the control group(P＜0.05);the exhaust time,pain duration,drainage time in the experimental group were significantly lower than those in the control group(P＜0.05),and the incidence rate of postoperative complications in the experimental group was significantly lower than that in the control group(P＜0.05).Conclusion The operative strategy by adopting laparoscopic cholecystectomy for treating acute cholecystitis complicating gallstones has good clinical value.
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Objective To explore the expression differences of long noncoding RNA (LncRNA) LOC100288637 in liver canc‐er ,the effect of LOC100288637 on liver cancer cell proliferation and the relevant mechanisms .Methods Based on the analysis of the GEO database data sets GSE58043 and GSE10694 ,we found that both LOC100288637 and hsa‐miR‐101‐3p has obvious expression differences in liver cancer tissues .RNA hybrid revealed the possibility of combination between LOC 100288637 and hsa‐miR‐101‐3p;RT‐PCR was performed to measure the expression level of LOC100288637 in tissues and cells ;fluorescence in situ hybridization was used to observe LOC100288637 localization in cells ;cell proliferation was determined by CCK8 experiment after LOC100288637 siRNA knock down .The expression of LOC100288637 in cells were measured after treated with hsa‐miR‐101‐3p mimics .Results Relative quantitative expression of LOC100288637 in liver cancer tissues group was significantly higher than that in no tumor tis‐sues group (P<0 .05);relative quantitative expression of LOC100288637 in liver cancer cell lines were significantly higher than that in normal liver cell line(P<0 .05) .LOC100288637 was located in both cytoplasm and nucleus of HepG2 cells ,and mainly in cy‐toplasm .Cell proliferation vitality of HepG2 reduced after treated with LOC100288637‐siRNA(P<0 .01) .Relative quantitative ex‐pression of LOC100288637 in HepG2 reduced after treated with hsa‐miR‐101‐3p mimics ( P< 0 .05 ) .Conclusion LncRNA LOC100288637 may play an important role in liver cancer development ,it can be down regulated by hsa‐miR‐101‐3p and affect the proliferation of liver cancer cells .
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Objective To evaluate the curative effect of different operative methods for duodenal diverticulum. Methods Articles of operations for duodenal diverticulum that were published between January 2000 to August 2006 were retrieved from China Journal Full-text Database, and the symptom remission rate and complication rate were underwent Meta-analysis. All data were analyzed by chi-square test. Results There was significant difference in symptom remission rate between Billroth Ⅱ subtotal gastrectomy, Roux-en-Y gastrojejunostomy and diverticulectomy, diverticulum inverting suture, sphincteroplasty of Oddi (χ~2= 21.269, 4.285, 12.914, 19.266, 3.938, P < 0.05). The difference in complication rate between Billroth Ⅱ subtotal gastrectomy, Roux-en-Y gastrojejunostomy and diverticulectomy, diverticulum inverting suture reached statistical significance (χ~2 =4.164, 9.166, 7.926,4.659, 4.858, P < 0.05). Conclusion For duodenal diverticulitis and the related complications which can not be effectively managed by internal medical treatment and with obvious symptom, Billroth Ⅱ subtotal gastrectomy and Roux-en-Y gastrojejunostumy are safe and effective.